Chemical Composition and Biological Activities of Trans-Himalayan Alga Spirogyra porticalis (Muell.) Cleve

نویسندگان

  • Jatinder Kumar
  • Priyanka Dhar
  • Amol B. Tayade
  • Damodar Gupta
  • Om P. Chaurasia
  • Dalip K. Upreti
  • Kiran Toppo
  • Rajesh Arora
  • M. R. Suseela
  • Ravi B. Srivastava
چکیده

The freshwater alga Spirogyra porticalis (Muell.) Cleve, a filamentous charophyte, collected from the Indian trans-Himalayan cold desert, was identified on the basis of morpho-anatomical characters. Extracts of this alga were made using solvents of varying polarity viz. n-hexane, acetonitrile, methanol and water. The antioxidant capacities and phenolic profile of the extracts were estimated. The methanol extract showing highest antioxidant capacity and rich phenolic attributes was further investigated and phytochemical profiling was conducted by gas chromatography-mass spectrometry (GC/MS) hyphenated technique. The cytotoxic activity of methanol extract was evaluated on human hepatocellular carcinoma HepG2 and colon carcinoma RKO cell lines. The anti-hypoxic effect of methanol extract of the alga was tested on in vivo animal system to confirm its potential to ameliorate oxidative stress. The antioxidant assays viz. ferric reducing antioxidant power (FRAP), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide (NO) radical scavenging capacities, β-carotene-linoleic acid bleaching property and lipid peroxidation exhibited analogous results, wherein the algal extracts showed significantly high antioxidant potential. The extracts were also found to possess high content of total proanthocyanidin, flavonoid and polyphenol. GC/MS analysis revealed the presence of thirteen chemotypes in the methanol extract representing different phytochemical groups like fatty acid esters, sterols, unsaturated alcohols, alkynes etc. with substantial phyto-pharmaceutical importance. The methanol extract was observed to possess anticancer activity as revealed from studies on HepG2 and RKO cell lines. In the present study, S. porticalis methanol extract also provided protection from hypoxia-induced oxidative stress and accelerated the onset of adaptative changes in rats during exposure to hypobaric hypoxia. The bioactive phytochemicals present in this trans-Himalayan alga are of enormous interest and can be utilized sustainably for discovery of novel drugs against oxidative stress.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Chemical Composition and Biological Activities of Essential Oil and Methanol Extract of Teucrium scordium

In this study, the chemical composition of the essential oil of Teucrium scordium was studied using capillary GC and GC/MS instruments. In addition, the antimicrobial and cytotoxic activities of the oil and methanol extract were evaluated by disc diffusion and MTT assays, respectively. Forty-three volatile components were identified from the oil of aerial parts, representing of 98....

متن کامل

Volatile Oil Constituents of the Eucalyptus viridis R. T. Baker and Eucalyptus oleosa F. Muell. Leaves from Iran

  Background: The genus Eucalyptus (family Myrtaceae) comprises well-known plants of over 600 species of trees. Although most of the plants are native to Australia, numerous species have been introduced to other parts of the world, including Iran, as economic and ornamental trees in forest trial provenances, where the plants have become source of important fast-growing hardwood trees and Eucaly...

متن کامل

Effects of Cadmium and Dimethoate on Some Biological and Biochemical Indices in Freshwater Green Algae, Spirogyra sp.

The present study investigates the influence of an organophosphorus pesticide, namely Dimethoate, and cadmium on biomarkers of the green alga, Spirogyra sp., in a 14-day experiment. For so doing, it has exposed Spirogyra sp. to 0.0, 100, 200, and 400 mg L-1 of Dimethoate and/or 1 mg L-1 of cadmium chloride (CdCl2) to observe a reduction in chlorophyll a and b level in Spirogyra sp., exposed to ...

متن کامل

Effects of Cadmium and Dimethoate on Some Biological and Biochemical Indices in Freshwater Green Algae, Spirogyra sp.

The present study investigates the influence of an organophosphorus pesticide, namely Dimethoate, and cadmium on biomarkers of the green alga, Spirogyra sp., in a 14-day experiment. For so doing, it has exposed Spirogyra sp. to 0.0, 100, 200, and 400 mg L-1 of Dimethoate and/or 1 mg L-1 of cadmium chloride (CdCl2) to observe a reduction in chlorophyll a and b level in Spirogyra sp., exposed to ...

متن کامل

Chemical Composition of the Essential Oils of Four Cultivated Eucalyptus Species in Iran as Medicinal Plants (E. microtheca, E. spathulata, E. largiflorens and E. torquata)

The leaves of four cultivated Eucalyptus species, Eucalyptus microtheca var. Microtheca F. Muell., Eucalyptus spathulata, Eucalyptus largiflorens and Eucalyptus torquata were collected in spring from Kashan and Isfahan provinces (central region of Iran). After drying the plant materials in shade, their essential oils were obtained by hydro-distillation. The oils were analyzed by capillary gas c...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 10  شماره 

صفحات  -

تاریخ انتشار 2015